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KMID : 1101420150470040251
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Korean Journal of Clinical Laboratory Science
2015 Volume.47 No. 4 p.251 ~ p.258
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Protective Effects of Curcumin on CCl4-Induced Hepatic Fibrosis with High Fat Diet in C57BL/6 Mice
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Jekal Seung-Joo
Min Byung-Woon
Park Ho
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Abstract
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Curcumin, a major polyphenolic compound of turmeric, is well known to prevent non-alcoholic steatohepatitis (NASH) related to obesity. The aim of the study was to investigate the effect of curcumin on hepatic fibrosis induced by carbon tetrachloride (CCl4) in obese mice. CCl4 was administrated in mice fed a normal diet (ND) or a high fat diet (HFD) for 7 weeks together with or without curcumin. It was conducted to examine for metabolic profiles, adipocyte size, and liver fibrosis by serum biochemistry, histology and immunohistochemistry. Also, Apoptosis of hepatic cells was determined by the TUNEL method. Treatment with curcumin significantly lowered the body weight, fasting glucose, serum AST and ALT, and decreased the adipocyte size, the number of macrophage and mast cells in adipose tissue, and collagen deposition in liver tissue in the HFD+CCl4 group compared with the findings of the HFD+CCl4 group. In contrast, treatment with curcumin on the ND+CCl4 group did not show a significant difference except the body weight and mast cell number when compared with the ND+CCl4 group. Furthermore, curcumin significantly reduced the number of parenchymal apoptotic cells, whereas it increased the number of non-parenchymal apoptotic cells, especially resembling an activated hepatic stellate cell in the liver. Taken together, this data suggests that curcumin might be an effective antifibrotic drug for the prevention of liver disease progression in obese mice. Thus, the development of curcumin as a therapy for obesity and liver fibrosis is supported.
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KEYWORD
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Curcumin, Obesity, Carbon tetrachloride, Hepatic fibrosis, Apoptosis
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